Develop an HPLC Method

Systematic development of a high-performance liquid chromatography method covering mode selection, column chemistry, mobile phase and gradient design, flow and temperature optimization, detector choice, and iterative refinement for non-volatile, thermally labile, or polar analytes.

When to Use

• Analyzing compounds that are non-volatile, thermally labile, or too polar for GC
• Developing a new HPLC-UV, HPLC-fluorescence, or LC-MS method from scratch
• Adapting a literature or pharmacopeial HPLC method to a different column or instrument
• Improving an existing method that suffers from poor resolution, long run times, or sensitivity issues
• Selecting the appropriate chromatographic mode (reversed-phase, HILIC, ion-exchange, SEC, chiral)

Inputs

Required

• Target analytes: Compound names, structures, molecular weights, pKa values, logP/logD
• Sample matrix: Formulation, biological fluid, environmental extract, or neat solution
• Performance targets: Required resolution, detection limits, quantitation range