bio-workflows-scrnaseq-pipeline
SKILL.md
Version Compatibility
Reference examples tested with: Cell Ranger 8.0+, ggplot2 3.5+, numpy 1.26+, scanpy 1.10+
Before using code patterns, verify installed versions match. If versions differ:
- Python:
pip show <package>thenhelp(module.function)to check signatures - R:
packageVersion('<pkg>')then?function_nameto verify parameters
If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.
Single-Cell RNA-seq Pipeline
"Analyze my single-cell RNA-seq data from counts to cell types" → Orchestrate QC filtering, normalization (scanpy/Seurat), batch integration (scVI/Harmony), clustering, marker detection, cell type annotation, and trajectory inference.
Complete workflow from 10X Genomics Cell Ranger output to annotated cell types.