binding-characterization

Installation
SKILL.md

Binding Characterization: SPR and BLI

SPR vs BLI Decision Matrix

Factor Choose SPR Choose BLI
Sensitivity Small molecules, fragments (<500 Da) Large complexes, antibodies
Throughput Low-medium (serial) High (96-well parallel)
Sample purity Required (clogs fluidics) Tolerates crude lysates
Kinetic resolution Higher (better for fast kinetics) Lower
Mass transport More sensitive (may distort kon) Less sensitive
Maintenance High (fluidics system) Low (dip-and-read)
Sample consumption Higher (continuous flow) Lower
Cost per experiment Lower chip cost, higher run cost Higher tip cost, lower run cost

Key differences

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First Seen
Jan 21, 2026