Source: https://github.com/aipoch/medical-research-skills

Differential Expression Gene Screening Analysis (Volcano Plot & Clustered Heatmap)

When to Use

Use this skill when you need a reproducible two-group DEG workflow on a bulk expression matrix and want:

• a full differential expression table
• a filtered DEG table
• a volcano plot
• a clustered heatmap of top differential genes

Typical requests include:

• compare case vs control samples with limma
• screen upregulated and downregulated genes from a normalized expression matrix
• generate a DEG table with volcano and heatmap outputs from bulk transcriptome data

Out of Scope