WGBS Differential Methylation with metilene

Overview

• Refer to the Inputs & Outputs section to check available inputs and design the output structure.
• Always prompt user for which columns in the BED files are methylation fraction/percent. Never decide by yourself.
• Convert heterogeneous inputs to a per‑sample 4‑column Metilene table (chrom, start, end, methylation_fraction). Sort the BED files after conversion.
• Generate the merged bed file as the input of metilene.
• Run metilene: call DMRs and DMCs with tunable parameters
• Visualize: quick plots (Δmethylation vs –log10(q), length histograms).

Inputs & Outputs

Inputs