Bio Interdomain HGT

Detect, polarize, and confirm horizontal gene transfer between a query genome (virus, MAG, isolate, or bin) and other domains of life. Built for the common asymmetric case where the query is well annotated but the comparison set is genome-only (proteins missing). Pairs with /bio-annotation (homology/taxonomy), /bio-phylogenomics (trees), /bio-viromics (viral classification), and /bio-fasta-database-curator (building the arbiter database).

Instructions

Run the steps in order; capture outputs and provenance at each step. Steps 0 (database gate) and 5 (frame-aware context guard on eukaryotic DNA) are the ones most often skipped and most often responsible for wrong conclusions.

Use the versioned evidence driver after the homology, context, and tree tools have produced their normalized TSVs: