bio-single-cell-batch-integration
Installation
SKILL.md
Version Compatibility
Reference examples tested with: scanpy 1.10+, Seurat 5.0+, scvi-tools 1.1+, harmonypy 0.0.10+
Before using code patterns, verify installed versions match. If versions differ:
- Python:
pip show <package>thenhelp(module.function)to check signatures - R:
packageVersion('<pkg>')then?function_nameto verify parameters
If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.
Batch Integration
"Integrate my batches" -> Learn a shared low-dimensional representation that mixes technical batches while preserving biological cell states, then cluster and visualize on it.
- Python:
sce.pp.harmony_integrate,scvi.model.SCVI,sce.pp.bbknn,scanorama - R:
RunHarmony,IntegrateLayers(Seurat v5),fastMNN(batchelor)