opentrons-integration
Opentrons Integration — Lab Automation
Overview
Opentrons provides a Python-based Protocol API (v2) for programming OT-2 and Flex liquid handling robots. Protocols are structured Python files with metadata and a run() function that controls pipettes, labware, and hardware modules. All protocols can be simulated locally before running on physical hardware.
When to Use
- Automating liquid handling workflows (pipetting, mixing, distributing)
- Writing PCR setup protocols with thermocycler control
- Performing serial dilutions across plates
- Replicating plates or reformatting between plate types
- Controlling hardware modules (temperature, magnetic, heater-shaker, thermocycler)
- Setting up multi-channel pipetting for 96-well plate operations
- Simulating protocols before running on the robot
- For multi-vendor automation (Hamilton, Beckman, etc.), use pylabrobot instead
- For flow cytometry analysis of automated experiment results, use flowio/flowkit
Prerequisites
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