Opentrons Python API v2

Overview

The Opentrons Python API v2 enables laboratory automation through Python protocols that control liquid handling robots (OT-2 and Flex). Write protocols that automate pipetting, temperature control, magnetic separation, plate reading, and complex multi-step workflows with reproducible precision.

Core value: Transform manual, error-prone pipetting workflows into automated, reproducible protocols. A 100-line protocol can replace hours of manual work with precise, documented liquid handling.

When to Use

Use the Opentrons skill when:

• Writing automated liquid handling protocols
• Setting up serial dilutions, plate replication, or reagent distribution
• Integrating temperature control, magnetic separation, or plate reading into workflows
• Adapting OT-2 protocols for Opentrons Flex
• Troubleshooting protocol execution or hardware integration
• Optimizing pipetting parameters (flow rates, tip positions, mixing)