Opentrons Thermocycler Module

Overview

The Opentrons Thermocycler Module automates PCR thermal cycling with precise, independent control of block temperature (4-99°C) and heated lid (37-110°C). Execute complex temperature profiles with automatic cycling, integrate with liquid handling for complete PCR setup automation, and use auto-sealing lids (GEN2) for walk-away workflows.

Core value: Fully automate PCR setup and cycling. Load samples, dispense reagents, seal plates (GEN2), run thermal profiles, and collect products—all without manual intervention.

When to Use

Use the Thermocycler skill when:

• Running PCR amplification protocols
• Performing qPCR or RT-PCR reactions
• Automating DNA/RNA thermal cycling workflows
• Executing multi-step temperature incubations
• Integrating thermal cycling with automated liquid handling
• Setting up high-throughput PCR screening