Opentrons Integration

Overview

Create production-minded Python Protocol API v2 protocols for Opentrons Flex and OT-2. This skill covers protocol structure, hardware and deck configuration, liquid handling, runtime customization, module control, simulation, and safe deployment.

The verified baseline as of 2026-07-23 is:

• opentrons==9.1.1 for reproducible Flex simulation.
• opentrons==9.0.0 for local OT-2 API 2.28 compatibility simulation.
• Flex supports API levels 2.15 through 2.29 on current software.
• OT-2 supports API levels 2.0 through 2.28 on current software.
• API 2.29 is Flex-only at this baseline. Do not put 2.29 in an OT-2 protocol.