QIIME 2 paired-end 16S amplicons

Use for a demultiplexed, paired-end 16S assay with known primers, quality encoding, expected insert length, and sample metadata. This bounded workflow imports reads, removes 5′ primers, denoises with DADA2, classifies ASVs using an explicitly supplied classifier, and retains .qza/.qzv provenance. Do not treat read counts as absolute cell counts or taxonomy assignments as strain identification.

Establish the assay before running