Protein Assembly Skill

This skill provides structured guidance for designing fusion protein gBlock sequences that combine multiple protein components (antibody fragments, fluorescent proteins, enzyme domains) into a single optimized DNA construct.

When to Use This Skill

This skill applies to tasks that involve:

• Designing fusion proteins from multiple sources (PDB, plasmids, protein databases)
• Creating gBlock sequences with specific linker requirements
• Codon optimization for GC content constraints
• Combining fluorescent proteins with specific excitation/emission wavelengths
• Assembling multi-domain proteins with N-terminal methionine removal

Structured Approach

Phase 1: Information Gathering and Cataloging

Objective: Collect ALL required sequence data before any design work begins.