tooluniverse-crispr-screen-analysis
ToolUniverse CRISPR Screen Analysis
Comprehensive skill for analyzing CRISPR-Cas9 genetic screens to identify essential genes, synthetic lethal interactions, and therapeutic targets through robust statistical analysis and pathway enrichment.
Overview
CRISPR screens enable genome-wide functional genomics by systematically perturbing genes and measuring fitness effects. This skill provides an 8-phase workflow for:
- Processing sgRNA count matrices
- Quality control and normalization
- Gene-level essentiality scoring (MAGeCK-like and BAGEL-like approaches)
- Synthetic lethality detection
- Pathway enrichment analysis
- Drug target prioritization with DepMap integration
- Integration with expression and mutation data
Core Workflow
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