Enzyme Kinetics (Michaelis-Menten)

Turn substrate concentration vs initial velocity data into Km, Vmax, kcat, and catalytic efficiency — and classify an inhibitor's mechanism.

The Michaelis-Menten model: v = Vmax·[S] / (Km + [S]).

When to use this

• You measured initial reaction rates at several substrate concentrations.
• You need Km (substrate affinity), Vmax, kcat (turnover number), or kcat/Km.
• You have ±inhibitor velocity data and want to classify the inhibition mode + Ki.

For published kinetic constants (someone else's Km/kcat), use the BRENDA tools instead — this skill is for analyzing your own measured data.

Step 1 — Prepare the data