Enzyme Kinetics (Michaelis-Menten)

Turn substrate concentration vs initial velocity data into Km, Vmax, kcat, and catalytic efficiency — and classify an inhibitor's mechanism.

The Michaelis-Menten model: v = Vmax·[S] / (Km + [S]).

When to use this

• You measured initial reaction rates at several substrate concentrations.
• You need Km (substrate affinity), Vmax, kcat (turnover number), or kcat/Km.
• You have ±inhibitor velocity data and want to classify the inhibition mode + Ki.

For published kinetic constants (someone else's Km/kcat), use the BRENDA tools or SABIO_RK_search_reactions instead — this skill is for analyzing your own measured data.

Looking up published constants instead of fitting your own (SABIO-RK)

SABIO_RK_search_reactions searches >200,000 literature-curated kinetic entries (Km, kcat, Vmax, Ki) by EC number, enzyme name, substrate, organism, or product — no authentication required. A second source alongside BRENDA; check both if a value seems off, since coverage and curation differ.