tooluniverse-protein-lof-mechanism
Installation
SKILL.md
Protein LoF Mechanism Synthesis
For a single missense variant, integrate 5 independent computational signals to propose a specific loss-of-function mechanism. Each signal answers a different question:
| Signal | Tool | Answers |
|---|---|---|
| Pathogenicity | AlphaMissense_get_variant_score |
"Is this variant damaging?" |
| Structural context | alphafold_get_prediction |
"Is the mutation in a folded vs disordered region?" |
| Sequence likelihood | ESM_score_sequence |
"Is the substitution evolutionarily plausible?" |
| Feature disruption | ESM_explain_variant_mechanism (or ESM_score_variant_sae_disruption + ESM_describe_sae_feature for raw control) |
"Which biological feature breaks?" |
| Stability | DynaMut2_predict_stability |
"Does the protein still fold correctly?" |