Opentrons Vision Validation

Goal

This is the Opentrons OT-2 adapter for the environment-neutral puda-machine-vision-validation skill. Load the generic gate first, then use this adapter for OT-2-specific deck slots, labware definitions, pipette mounts, trash, and A1–H12 tip/well checks. Camera calibration, slot polygons, endpoints, and current-scene evidence must remain scoped to the active environment (BEARS, IMRE, NTU, or another site).

Prevent Opentrons OT-2 runs from starting with the wrong physical deck setup. Before a physical Opentrons run in the active PUDA environment, capture a fresh image from that environment's configured camera, verify the slots used by the protocol, identify visible labware/items, and stop for user confirmation when anything is uncertain or mismatched.

This is a physical safety gate. PUDA protocol validation checks command structure; it does not prove the robot deck contains the expected labware.

When to Use

Use this skill before running any Opentrons protocol that depends on deck setup, including:

• liquid transfer, mixing, aspiration, dispense, or plate workflows
• protocols with tipracks, source plates, destination plates, reservoirs, vials, or modules
• colour-mixing or viscosity workflows that generate/run OT-2 protocols
• any user request that names deck slots or labware placement