basic-caching-evaluation
Installation
SKILL.md
BaSiC Caching Evaluation
Experiment Overview
| Item | Details |
|---|---|
| Date | 2025-12-11 |
| Goal | Evaluate whether caching BaSiC illumination profiles across cycles/channels improves processing speed |
| Environment | CuPy GPU acceleration, multiplex immunofluorescence data |
| Status | FAILED - Caching NOT recommended |
Context
BaSiC (Background and Shading Correction) computes flatfield and darkfield correction profiles for each image stack. The hypothesis was that similar channels across cycles might share illumination profiles, allowing cached profiles to skip computation.
CRITICAL FINDING: DO NOT CACHE
Caching BaSiC profiles causes 15-20% intensity errors in sparse markers.