Version Compatibility

Reference examples tested with: BioPython 1.83+, NCBI BLAST+ 2.15+

Before using code patterns, verify installed versions match. If versions differ:

• Python: pip show <package> then help(module.function) to check signatures

If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.

BLAST Searches

"BLAST this sequence against NCBI" → Submit a query sequence to NCBI BLAST servers, retrieve XML results, and parse hits with E-values and identity scores.

• Python: NCBIWWW.qblast() + NCBIXML.read() (BioPython)
• CLI: blastn -remote -db nt -query seq.fa (BLAST+)
• R: blast() (rBLAST, local only)

Run BLAST searches against NCBI databases using Biopython's Bio.Blast module.