bio-clip-seq-binding-site-annotation
Installation
SKILL.md
Version Compatibility
Reference examples tested with: bedtools 2.31+, pandas 2.2+
Before using code patterns, verify installed versions match. If versions differ:
- Python:
pip show <package>thenhelp(module.function)to check signatures - R:
packageVersion('<pkg>')then?function_nameto verify parameters - CLI:
<tool> --versionthen<tool> --helpto confirm flags
If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.
Binding Site Annotation
"Annotate where my RBP binds in transcripts" → Map CLIP-seq peaks to genomic features (3'UTR, 5'UTR, CDS, introns, ncRNAs) to characterize RNA-binding protein target regions.
- R:
ChIPseeker::annotatePeak()with transcript annotation databases - CLI:
bedtools intersectwith gene model BED files