bio-crispr-screens-base-editing-analysis
Installation
SKILL.md
Version Compatibility
Reference examples tested with: CRISPResso2 2.2+, pandas 2.2+
Before using code patterns, verify installed versions match. If versions differ:
- Python:
pip show <package>thenhelp(module.function)to check signatures - CLI:
<tool> --versionthen<tool> --helpto confirm flags
If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.
Base Editing Analysis
"Analyze my base editing outcomes" → Quantify base editing efficiency, bystander edits, and indel frequencies from amplicon sequencing data for CBE, ABE, and prime editing experiments.
- CLI:
CRISPResso --fastq_r1 reads.fq --amplicon_seq ATGC --base_editor_output
CRISPResso2 for Base Editing
Goal: Quantify base editing efficiency and bystander edits from amplicon sequencing.