bio-crispr-screens-crispresso-editing
Installation
SKILL.md
Version Compatibility
Reference examples tested with: CRISPResso2 2.2+, pandas 2.2+
Before using code patterns, verify installed versions match. If versions differ:
- Python:
pip show <package>thenhelp(module.function)to check signatures - CLI:
<tool> --versionthen<tool> --helpto confirm flags
If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.
CRISPResso2 Editing Analysis
"Quantify CRISPR editing from my amplicon data" → Analyze amplicon sequencing to measure indel frequencies, HDR efficiency, and frameshift rates from CRISPR gene editing experiments.
- CLI:
CRISPResso --fastq_r1 reads.fq --amplicon_seq ATGC --guide_seq GUIDE
Basic Analysis
Goal: Quantify CRISPR editing outcomes from amplicon sequencing of a single target site.