Version Compatibility

Reference examples tested with: DESeq2 1.42+

Before using code patterns, verify installed versions match. If versions differ:

• R: packageVersion('<pkg>') then ?function_name to verify parameters

If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.

Sample Size Estimation

"How many samples do I need for my experiment?" → Estimate required biological replicates per group for a target power level given expected effect sizes and variability.

• R: ssizeRNA::ssizeRNA_single(), DESeq2 pilot dispersion estimates
• scRNA-seq: powsimR::simulateDE()

RNA-seq Sample Size