Version Compatibility

Reference examples tested with: flowCore 2.14+, ggplot2 3.5+

Before using code patterns, verify installed versions match. If versions differ:

• R: packageVersion('<pkg>') then ?function_name to verify parameters

If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.

Bead Normalization

"Normalize my CyTOF data using beads" → Correct instrument signal drift over acquisition time using EQ calibration bead intensities for consistent measurements across runs.

• R: CATALYST::normCytof() for EQ bead normalization

CyTOF EQ Bead Normalization

Goal: Identify EQ normalization bead events in CyTOF data for signal calibration.