Version Compatibility

Reference examples tested with: flowCore 2.14+, ggplot2 3.5+

Before using code patterns, verify installed versions match. If versions differ:

• R: packageVersion('<pkg>') then ?function_name to verify parameters

If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.

Doublet Detection

"Remove doublets from my flow cytometry data" → Detect and filter out cell aggregates using FSC-A/FSC-H gating or computational methods before clustering or quantitative analysis.

• R: flowCore rectangular gates on FSC-A vs FSC-H

FSC-A vs FSC-H Gating (Standard Method)