Version Compatibility

Reference examples tested with: flowCore 2.14+

Before using code patterns, verify installed versions match. If versions differ:

• R: packageVersion('<pkg>') then ?function_name to verify parameters

If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.

Gating Analysis

"Gate my flow cytometry data to identify cell populations" → Define cell populations through manual or automated gating strategies using rectangular, polygon, or data-driven gates in a hierarchical framework.

• R: flowWorkspace::gs_add_gating_method(), openCyto::gating() for automated gating

Manual Rectangular Gates

library(flowCore)