bio-imaging-mass-cytometry-data-preprocessing
Installation
SKILL.md
Version Compatibility
Reference examples tested with: anndata 0.10+, numpy 1.26+, pandas 2.2+, scanpy 1.10+, scipy 1.12+, steinbock 0.16+
Before using code patterns, verify installed versions match. If versions differ:
- Python:
pip show <package>thenhelp(module.function)to check signatures - CLI:
<tool> --versionthen<tool> --helpto confirm flags
If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.
IMC Data Preprocessing
"Preprocess my imaging mass cytometry data" → Load MCD files, apply hot pixel removal, channel cropping, and signal normalization to prepare multiplexed images for segmentation and analysis.
- CLI:
steinbock preprocessfor automated IMC preprocessing pipeline