Version Compatibility

Reference examples tested with: NCBI BLAST+ 2.15+

Before using code patterns, verify installed versions match. If versions differ:

• Python: pip show <package> then help(module.function) to check signatures
• CLI: <tool> --version then <tool> --help to confirm flags

If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.

Local BLAST

"Run a BLAST search against my custom database" → Build a local BLAST database and search it with query sequences, returning tabular results with identity and e-value.

• CLI: makeblastdb, blastn/blastp (NCBI BLAST+)
• Python: subprocess wrapper for BLAST+

Run BLAST searches locally using NCBI BLAST+ command-line tools.