Version Compatibility

Reference examples tested with: BioPython 1.83+, pandas 2.2+, primer3-py 2.0+

Before using code patterns, verify installed versions match. If versions differ:

• Python: pip show <package> then help(module.function) to check signatures

If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.

PCR Primer Design

"Design primers for this sequence" → Given a template sequence and constraints (product size, Tm, GC%), find ranked primer pairs that amplify the target region.

• Python: primer3.design_primers() (primer3-py)
• CLI: primer3_core (Primer3)

Design PCR primers using primer3-py, the Python binding for Primer3.

Required Imports