Version Compatibility

Reference examples tested with: pandas 2.2+, primer3-py 2.0+

Before using code patterns, verify installed versions match. If versions differ:

• Python: pip show <package> then help(module.function) to check signatures

If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.

Primer Validation

Check primers for secondary structures, dimers, and other issues using primer3-py.

"Validate a primer pair" → Check for hairpins, self-dimers, heterodimers, and 3' stability using thermodynamic calculations.

• Python: primer3.calc_hairpin(), primer3.calc_homodimer(), primer3.calc_heterodimer() (primer3-py)

Required Imports