Version Compatibility

Reference examples tested with: BioPython 1.83+, samtools 1.19+

Before using code patterns, verify installed versions match. If versions differ:

• Python: pip show <package> then help(module.function) to check signatures

If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.

Reverse Complement

Generate complementary and reverse complementary sequences using Biopython.

"Get the reverse complement" → Produce the 5'-to-3' sequence of the opposite strand.

• Python: seq.reverse_complement() (BioPython Seq)
• CLI: samtools faidx ref.fa region --reverse-complement

Required Import