Version Compatibility

Reference examples tested with: BioPython 1.83+, samtools 1.19+

Before using code patterns, verify installed versions match. If versions differ:

• Python: pip show <package> then help(module.function) to check signatures

If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.

Sequence Properties

Calculate physical and chemical properties of biological sequences using Biopython.

"Calculate GC content" → Compute the fraction of G+C bases in a nucleotide sequence.

• Python: gc_fraction(seq) (BioPython SeqUtils)

"Analyze protein properties" → Compute MW, pI, stability, hydrophobicity from an amino acid sequence.

• Python: ProteinAnalysis(str_seq) (BioPython ProtParam)