Version Compatibility

Reference examples tested with: BioPython 1.83+, samtools 1.19+

Before using code patterns, verify installed versions match. If versions differ:

• Python: pip show <package> then help(module.function) to check signatures

If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.

Sequence Statistics

"Calculate N50 and other assembly statistics" → Compute sequence count, length distribution, N50/L50, GC content, and nucleotide composition for FASTA datasets.

• Python: SeqIO.parse(), gc_fraction() (BioPython)

Calculate comprehensive statistics for sequence datasets using Biopython.

Required Imports