TopExpressingGenesOfAllCells Process Configuration

Purpose

Identifies and visualizes the top expressing genes per cluster across ALL cells (before T/B cell selection), followed by pathway enrichment analysis. Provides initial overview of all cell populations by highlighting the most highly expressed genes and their biological functions.

When to Use

• After: SeuratClusteringOfAllCells process
• Before: TOrBCellSelection (this is a pre-selection analysis)
• Use cases:
  • Quick overview of ALL cell populations before separation
  • Initial assessment of broad cell type signatures
  • Understanding overall cell composition before T/B selection
  • Pathway enrichment on cell type markers before detailed analysis
  • Quality check for unexpected cell types
  • Complementary to ClusterMarkersOfAllCells for complete pre-selection profiling
• Optional process: Enable only when pre-selection analysis is needed

Configuration Structure