ToolUniverse CRISPR Screen Analysis

Comprehensive skill for analyzing CRISPR-Cas9 genetic screens to identify essential genes, synthetic lethal interactions, and therapeutic targets through robust statistical analysis and pathway enrichment.

Overview

CRISPR screens enable genome-wide functional genomics by systematically perturbing genes and measuring fitness effects. This skill provides an 8-phase workflow for:

• Processing sgRNA count matrices
• Quality control and normalization
• Gene-level essentiality scoring (MAGeCK-like and BAGEL-like approaches)
• Synthetic lethality detection
• Pathway enrichment analysis
• Drug target prioritization with DepMap integration
• Integration with expression and mutation data

Core Workflow

Phase 1: Data Import & sgRNA Count Processing