Bioinformatics Visualization

iTOL Dataset Formats and Troubleshooting

Choosing the Right Dataset Type

DATASET_BINARY (Recommended for markers/symbols):

• More reliable than DATASET_SYMBOL
• All species must be listed with binary values (0 or 1)
• Simpler format, better iTOL compatibility
• Use for: presence/absence markers, technology indicators, categorical highlights

Format example:

DATASET_BINARY
SEPARATOR TAB