Version Compatibility

Reference examples tested with: scanpy 1.10+, Seurat 5.0+, anndata 0.10+

Before using code patterns, verify installed versions match. If versions differ:

• Python: pip show <package> then help(module.function) to check signatures
• R: packageVersion('<pkg>') then ?function_name to verify parameters

If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.

Single-Cell Data I/O

"Load my 10X data" -> Parse a Cell Ranger matrix into an annotated object (cells, genes, counts, metadata).

• Python: sc.read_10x_mtx() / sc.read_10x_h5() -> AnnData
• R: Read10X() / Read10X_h5() -> CreateSeuratObject()

Governing Principle