ToolUniverse Immune Repertoire Analysis

Comprehensive skill for analyzing T-cell receptor (TCR) and B-cell receptor (BCR) repertoire sequencing data to characterize adaptive immune responses, clonal expansion, and antigen specificity.

Overview

Adaptive immune receptor repertoire sequencing (AIRR-seq) enables comprehensive profiling of T-cell and B-cell populations through high-throughput sequencing of TCR and BCR variable regions. This skill provides an 8-phase workflow for:

• Clonotype identification and tracking
• Diversity and clonality assessment
• V(D)J gene usage analysis
• CDR3 sequence characterization
• Clonal expansion and convergence detection
• Epitope specificity prediction
• Integration with single-cell phenotyping
• Longitudinal repertoire tracking

Core Workflow

Phase 1: Data Import & Clonotype Definition